Issue

Vol. 10 No. 4 (2018)

Visualizing Photodynamic Therapy in Transgenic Zebrafish Using Organic Nanoparticles with Aggregation-Induced Emission

https://doi.org/10.1007/s40820-018-0214-4
Purnima Naresh Manghnani
Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore 117585, Singapore
Wenbo Wu
Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore 117585, Singapore
Shidang Xu
Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore 117585, Singapore
Fang Hu
Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore 117585, Singapore
Cathleen Teh
Institute of Molecular and Cell Biology, Proteos Building, Biopolis Drive, Singapore 138673, Singapore
Bin Liu
Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore 117585, Singapore

Corresponding Author: Bin Liu

cheliub@nus.edu.sg

Nano-Micro Letters, Vol. 10 No. 4 (2018), Article Number: 61

Abstract

Photodynamic therapy (PDT) employs accumulation of photosensitizers (PSs) in malignant tumor tissue followed by the light-induced generation of cytotoxic reactive oxygen species to kill the tumor cells. The success of PDT depends on optimal PS dosage that is matched with the ideal power of light. This in turn depends on PS accumulation in target tissue and light administration time and period. As theranostic nanomedicine is driven by multifunctional therapeutics that aim to achieve targeted tissue delivery and image-guided therapy, fluorescent PS nanoparticle (NP) accumulation in target tissues can be ascertained through fluorescence imaging to optimize the light dose and administration parameters. In this regard, zebrafish larvae provide a unique transparent in vivo platform to monitor fluorescent PS bio-distribution and their therapeutic efficiency. Using fluorescent PS NPs with unique aggregation-induced emission characteristics, we demonstrate for the first time the real-time visualization of polymeric NP accumulation in tumor tissue and, more importantly, the best time to conduct PDT using transgenic zebrafish larvae with inducible liver hyperplasia as an example.


Highlights:


1 The key novelty of this work is the creation of an in vivo model that can be used to effectively visualize image-guided photodynamic therapy. This allows fast screening of the performance of photosensitizers and their formulations.
2 Transparent zebrafish larvae provide a visual understanding of bio-distribution of nanoparticles, thereby enabling smarter formulation strategies.

Keywords

Nanomedicine Photodynamic therapy Transgenic zebrafish Aggregation-induced emission Organic nanoparticles
Manghnani, Purnima Naresh, Wenbo Wu, Shidang Xu, Fang Hu, Cathleen Teh, and Bin Liu. 2018. “Visualizing Photodynamic Therapy in Transgenic Zebrafish Using Organic Nanoparticles With Aggregation-Induced Emission”. Nano-Micro Letters 10 (4):61. https://doi.org/10.1007/s40820-018-0214-4.
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