Issue

Vol. 13 (2021)

FLIM as a Promising Tool for Cancer Diagnosis and Treatment Monitoring

https://doi.org/10.1007/s40820-021-00653-z
Yuzhen Ouyang
Hunan Provincial Tumor Hospital and the Affiliated Tumor Hospital of Xiangya Medical School, Central South University, Changsha 410013, Hunan, People’s Republic of China
Yanping Liu
School of Physics and Electronics, Hunan Key Laboratory for Super‑Microstructure and Ultrafast Process, Central South University, 932 South Lushan Road, Changsha 410083, Hunan, People’s Republic of China
Zhiming M. Wang
Institute of Fundamental and Frontier Sciences, University of Electronic Science and Technology of China, Chengdu 610054, Sichuan, People’s Republic of China
Zongwen Liu
School of Chemical and Biomolecular Engineering, The University of Sydney, Sydney, NSW 2006, Australia
Minghua Wu
Hunan Provincial Tumor Hospital and the Affiliated Tumor Hospital of Xiangya Medical School, Central South University, Changsha 410013, Hunan, People’s Republic of China

Corresponding Author: Minghua Wu

wuminghua554@aliyun.com

Nano-Micro Letters, Vol. 13 (2021), Article Number: 133

Abstract

Fluorescence lifetime imaging microscopy (FLIM) has been rapidly developed over the past 30 years and widely applied in biomedical engineering. Recent progress in fluorophore-dyed probe design has widened the application prospects of fluorescence. Because fluorescence lifetime is sensitive to microenvironments and molecule alterations, FLIM is promising for the detection of pathological conditions. Current cancer-related FLIM applications can be divided into three main categories: (i) FLIM with autofluorescence molecules in or out of a cell, especially with reduced form of nicotinamide adenine dinucleotide, and flavin adenine dinucleotide for cellular metabolism research; (ii) FLIM with Förster resonance energy transfer for monitoring protein interactions; and (iii) FLIM with fluorophore-dyed probes for specific aberration detection. Advancements in nanomaterial production and efficient calculation systems, as well as novel cancer biomarker discoveries, have promoted FLIM optimization, offering more opportunities for medical research and applications to cancer diagnosis and treatment monitoring. This review summarizes cutting-edge researches from 2015 to 2020 on cancer-related FLIM applications and the potential of FLIM for future cancer diagnosis methods and anti-cancer therapy development. We also highlight current challenges and provide perspectives for further investigation.


Highlights:


1 Fluorescence lifetime imaging microscopy (FLIM) applications for cancer diagnosis and treatment monitoring combined with reduced form of nicotinamide adenine dinucleotide, Förster resonance energy transfer (FRET), and biosensors are reviewed.
2 Principles of FLIM, previous clinical applications, and development history are introduced.
3 The current challenges and prospects for the potential of FLIM for cancer diagnosis and promotion of the effect of anti-cancer treatment are discussed.

Keywords

Fluorescence lifetime imaging microscopy Förster resonance energy transfer Reduced form of nicotinamide adenine dinucleotide Biosensors Cancer
Ouyang, Yuzhen, Yanping Liu, Zhiming M. Wang, Zongwen Liu, and Minghua Wu. 2021. “FLIM As a Promising Tool for Cancer Diagnosis and Treatment Monitoring”. Nano-Micro Letters 13 (June):133. https://doi.org/10.1007/s40820-021-00653-z.
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