Issue

Vol. 5 No. 3 (2013)

One Step Quick Detection of Cancer Cell Surface Marker by Integrated NiFe-based Magnetic Biosensing Cell Cultural Chip

https://doi.org/10.1007/BF03353752
Chenchen Bao
Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Research Institute of Micro/Nano Science and Technology, Shanghai Jiao Tong University, Shanghai 200240, China
Lei Chen
Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Research Institute of Micro/Nano Science and Technology, Shanghai Jiao Tong University, Shanghai 200240, China
Tao Wang
Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Research Institute of Micro/Nano Science and Technology, Shanghai Jiao Tong University, Shanghai 200240, China
Chong Lei
Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Research Institute of Micro/Nano Science and Technology, Shanghai Jiao Tong University, Shanghai 200240, China
Furong Tian
FOCAS Research Institute, Dublin Institute of Technology, Kevin Street, Dublin 8, Ireland
Daxiang Cui
Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Research Institute of Micro/Nano Science and Technology, Shanghai Jiao Tong University, Shanghai 200240, China
Yong Zhou
Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Research Institute of Micro/Nano Science and Technology, Shanghai Jiao Tong University, Shanghai 200240, China

Corresponding Author: Yong Zhou

dxcui@sjtu.edu.cn

Nano-Micro Letters, Vol. 5 No. 3 (2013), Article Number: 213-222

Abstract

RGD peptides has been used to detect cell surface integrin and direct clinical effective therapeutic drug selection. Herein we report that a quick one step detection of cell surface marker that was realized by a specially designed NiFe-based magnetic biosensing cell chip combined with functionalized magnetic nanoparticles. Magnetic nanoparticles with 20-30 nm in diameter were prepared by coprecipitation and modified with RGD-4C, and the resultant RGD-functionalized magnetic nanoparticles were used for targeting cancer cells cultured on the NiFe-based magnetic biosensing chip and distinguish the amount of cell surface receptor-integrin. Cell lines such as Calu3, Hela, A549, CaFbr, HEK293 and HUVEC exhibiting different integrin expression were chosen as test samples. Calu3, Hela, HEK293 and HUVEC cells were successfully identified. This approach has advantages in the qualitative screening test. Compared with traditional method, it is fast, sensitive, low cost, easy-operative, and needs very little human intervention. The novel method has great potential in applications such as fast clinical cell surface marker detection, and diagnosis of early cancer, and can be easily extended to other biomedical applications based on molecular recognition.

Keywords

Magnetic impedance Cancer cell Targeted detection Biosensing system Cell cultural chip
Bao, Chenchen, Lei Chen, Tao Wang, Chong Lei, Furong Tian, Daxiang Cui, and Yong Zhou. 2013. “One Step Quick Detection of Cancer Cell Surface Marker by Integrated NiFe-Based Magnetic Biosensing Cell Cultural Chip”. Nano-Micro Letters 5 (3):213-22. https://doi.org/10.1007/BF03353752.
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References
  1. C. Gui, X. Dai and D. Cui, “Advances of nanotech- nology applied to biosensors”, Nano Biomed. Eng. 3(4), 260–273 (2011). http://dx.doi.org/10.5101/nbe.v3i4.p260-273
  2. J. Chen, D. Chen, T. Yuan and X. Chen, “Microfluidic PCR Chips”, Nano Biomed. Eng. 3 (4), 203–210 (2011). http://dx.doi.org/10.5101/nbe.v3i4.p203-210
  3. R. Vijayakumar, S. Jagannathan, P. R. Gandhi and S. Chaansha, “Nanorobotics: a newer platform for molecular diagnose”, Nano Biomed. Eng. 3(3), 192–201 (2011). http://dx.doi.org/10.5101/nbe.v3i3.p192-201
  4. X. Zhang, L. Jiang, C. Zhang, D. Li, C. Wang, F. Gao and D. Cui, “A silicon dioxide modified magnetic nanoparticles labeled lateral flow strips for HBs antigen”, J. Biomed. Nanotechnol. 7(6), 776–781 (2011). http://dx.doi.org/10.1166/jbn.2011.1352
  5. R. Bashir, “BioMEMS: state-of-the-art in detection, opportunities and prospects”, Adv. Drug Deliv. Rev. 56(11), 1565–1586 (2004). http://dx.doi.org/10.1016/j.addr.2004.03.002.
  6. D. R. Baselt, G. U. Lee, M. Natesan, S.W. Metzger, P. E. Sheehan and R. J. Colton, “A biosensor based on magnetoresistance technology”, Biosens. Bioelectron. 13(7), 731–739 (1998). http://dx.doi.org/10.1016/S0956-5663(98)00037-2
  7. G. Kurlyandskaya and V. Levit, “Magnetic Dynabeads detection by sensitive element based on giant magnetoimpedance”, Biosens. Bioelectron. 20(8), 1611–1616 (2005). http://dx.doi.org/10.1016/j.bios.2004.04.027
  8. S. Baranov, “Magnetic models of cast amorphous microwires”, Surf. Eng. Appl. Electrochem. 47(4), 308–322 (2011). http://dx.doi.org/10.3103/S1068375511040028
  9. F. Blanc-Beguin, S. Nabily, J. Gieraltowski, A. Turzo, S. Querellou and P. Salaun, “Cytotoxicity and GMI bio-sensor detection of maghemite nanoparticles internalized into cells”, J. Magn. Magn. Mater. 321(3), 192–197 (2009). http://dx.doi.org/10.1016/j.jmmm.2008.08.104
  10. H. Yang, L. Chen, C. Lei, J. Zhang, D. Li, Z. M. Zhou, C. C. Bao, H. Y. Hu, X. Chen and F. Cui, “Giant magnetoimpedance-based microchannel system for quick and parallel genotyping of human papilloma virus type 16/18”, Appl. Phys. Lett. 97 (4), 043702-043702-3 (2010). http://dx.doi.org/10.1063/1.3467833
  11. X. Zhi, Q. Liu, X. Zhang, Y. Zhang, J. Feng and D. Cui, “Quick genotyping detection of HBV by giant magnetoresistive biochip combined with PCR and line probe assay”, Lab Chip. 12(4), 741 (2012). http://dx.doi.org/10.1039/c2lc20949g
  12. L. Chen, C.-C. Bao, H. Yang, D. Li, C. Lei, T. Wang, H.-Y. Hu, M. He, Y. Zhou and D.-X. Cui, “A prototype of giant magnetoimpedance-based biosensing system for targeted detection of gastric cancer cells”, Biosens. Bioelectron. 26(7), 3246–3253 (2011). http://dx.doi.org/10.1016/j.bios.2010.12.034
  13. G. Kurlyandskaya, “Giant magnetoimpedance for biosensing: Advantages and shortcomings”, J. Magn. Magn. Mater. 321(7), 659–662 (2009). http://dx.doi.org/10.1016/j.jmmm.2008.11.019
  14. L. Chen, Y. Zhou, C. Lei, Z. Zhou and W. Ding, “Giant magnetoimpedance effect in sputtered single layered NiFe film and meander NiFe/Cu/NiFe film”, J. Magn. Magn. Mater. 322(19), 2834–2839 (2010). http://dx.doi.org/10.1016/j.jmmm.2010.04.038
  15. S. Petersen, J. M. Alonso, A. Specht, P. Duodu, M. Goeldner and A. delCampo, “Phototriggering of Cell Adhesion by Caged Cyclic RGD Peptides”, Angew. Chem. Int. Ed. 47(17), 3192–3195 (2008). http://dx.doi.org/10.1002/anie.200704857
  16. X. Chen, “Multimodality imaging of tumor integrin alphavbeta3 expression”, Mini-Rev. Med. Chem. 6(2), 227 (2006). http://dx.doi.org/10.2174/138955706775475975
  17. H. D. Han, L. S. Mangala, J. W. Lee, M. M. K. Shahzad, H. S. Kim, D. Shen, E. J. Nam, E. M. Mora, R. L. Stone, C. Lu, S. J. Lee, J.W. Roh, A.M. Nick, G. Lopez-Berestein and A. K. Sood, “Targeted Gene Silencing Using RGD-Labeled Chitosan Nanoparticles”, Clin. Cancer Res. 16(15), 3910–3922 (2010). http://dx.doi.org/10.1158/1078-0432.ccr-10-0005.
  18. X. Montet, K. Montet-Abou, F. Reynolds, R. Weissleder and L. Josephson, “Nanoparticle imaging of integrins on tumor cells”, Neoplasia (New York, NY). 8(3), 214 (2006). http://dx.doi.org/10.1593/neo.05769
  19. Z. Li, P. Huang, X. Zhang, J. Lin, S. Yang, B. Liu, F. Gao, P. Xi, Q. Ren and D. Cui, “RGD- conjugated dendrimer-modified gold nanorods for in vivo tumor targeting and photothermal therapy”, Mol. Pharm. 7(1), 94–104 (2009). http://dx.doi.org/10.1021/mp9001415
  20. Y. Ye, S. Bloch, B. Xu and S. Achilefu, “Design, synthesis, and evaluation of near infrared fluorescent multimeric RGD peptides for targeting tumors”, J. Med. Chem. 49(7), 2268–2275 (2006). http://dx.doi.org/10.1021/jm050947h
  21. I. Schmid, C. H. Uittenbogaart, B. Keld and J. V. Giorgi, “A rapid method for measuring apoptosis and dual-color immunofluorescence by single laser flow cytometry”, J. Immunol. Methods. 170(2), 145 (1994). http://dx.doi.org/10.1016/0022-1759(94)90390-5
  22. C. Bao, N. Beziere, P. del Pino, B. Pelaz, G. Estrada, F. Tian, V. Ntziachristos, J. M. de la Fuente and D. Cui, “Gold nanoprisms as optoacoustic signal nanoamplifiers for in vivo bioimaging of gastrointestinal cancers”, Small 9(1), 68–74 (2013). http://dx.doi.org/10.1002/smll.201201779
  23. H. Hu, H. Yang, D. Li, K. Wang, J. Ruan, X. Zhang, J. Chen, C. Bao, J. Ji and D. Shi, “The potential of magnetic nanocluster and dual-functional protein-based strategy for noninvasive detection of HBV surface antibodies”, Analyst. 136(4), 679–683 (2010). http://dx.doi.org/10.1039/c0an00517g
  24. B. Pan, D. Cui, Y. Sheng, C. Ozkan, F. Gao, R. He, Q. Li, P. Xu and T. Huang, “Dendrimer-modified magnetic nanoparticles enhance efficiency of gene delivery system”, Cancer research. 67(17), 8156–8163 (2007). http://dx.doi.org/10.1158/0008-5472.CAN-06-4762
  25. G. Gao, P. Huang, Y. Zhang, K. Wang, W. Qin and D. Cui, “Gram scale synthesis of superparamagnetic Fe3O4 nanoparticles and fluid via a facile solvothermal route”, Crystengcomm. 13(6), 1782–1785 (2011). http://dx.doi.org/10.1039/c0ce00584c
  26. G. Gao, P. Huang, K. Wang, R. He and D. Cui, “Gramscale synthesis and shape evolution of micro-CaCO3”, Powder Technol. 205(1), 270–275 (2011). http://dx.doi.org/10.1016/j.powtec.2010.09.032
  27. H. Hu, H. Yang, P. Huang, D. Cui, Y. Peng, J. Zhang, F. Lu, J. Lian and D. Shi, “Unique role of ionic liquid in microwave-assisted synthesis of monodisperse magnetite nanoparticles”, Chem. Commun. 46(22), 3866–3868 (2010). http://dx.doi.org/10.1039/b927321b
  28. C. Zhang, M. Jugold, E. C. Woenne, T. Lammers, B. Morgenstern, M. M. Mueller, H. Zentgraf, M. Bock, M. Eisenhut and W. Semmler, “Specific targeting of tumor angiogenesis by RGD-conjugated ultrasmall superparamagnetic iron oxide particles using a clinical 1.5-T magnetic resonance scanner”, Cancer Res. 67(4), 1555–1562 (2007). http://dx.doi.org/10.1158/0008-5472.CAN-06-1668
  29. G. Thumshirn, U. Hersel, S. L. Goodman and H. Kessler, “Multimeric cyclic RGD peptides as poten- tial tools for tumor targeting: solid-phase peptide synthesis and chemoselective oxime ligation”, Chem. Eur. J. 9(12), 2717–2725 (2003). http://dx.doi.org/10.1002/chem.200204304
  30. E. D. Hay, “Collagen and other matrix glycoproteins in embryogenesis”, Cell biology of extracellular matrix 2, 419–462 (1991).
  31. J. Madri, “Endothelial cell-matrix interactions in hemostasis”, Progress in hemostasis and thrombosis. 6, 1 (1982).
  32. U. Saarialho-Kere, S. Kovacs, A. Pentland, J. Olerud, H. Welgus and W. Parks, “Cell-matrix interactions modulate interstitial collagenase expression by human keratinocytes actively involved in wound healing”, J. Clin. Invest. 92(6), 2858–2866 (1993). http://dx.doi.org/10.1172/JCI116906
  33. G. G. Vaday and O. Lider, “Extracellular matrix moieties, cytokines, and enzymes: dynamic effects on immune cell behavior and inflammation”, J. Leukoc. Biol. 67(2), 149–159 (2000).
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