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Vol. 9 No. 1 (2017)

Electrochemical Impedance Analysis of Biofunctionalized Conducting Polymer-Modified Graphene-CNTs Nanocomposite for Protein Detection

https://doi.org/10.1007/s40820-016-0108-2
Shobhita Singal
CSIR-National Physical Laboratory, Dr. K. S. Krishnan Road, New Delhi 110012, India
Avanish K. Srivastava
CSIR-National Physical Laboratory, Dr. K. S. Krishnan Road, New Delhi 110012, India
Rajesh Rajesh
CSIR-National Physical Laboratory, Dr. K. S. Krishnan Road, New Delhi 110012, India

Corresponding Author: Rajesh Rajesh

rajesh_csir@yahoo.com

Nano-Micro Letters, Vol. 9 No. 1 (2017), Article Number: 7

Abstract

We report an electrodeposited poly(pyrrole-co-pyrrolepropylic acid) copolymer modified electroactive graphene-carbon nanotubes composite deposited on a glassy carbon electrode to detect the protein antigen (cTnI). The copolymer provides pendant carboxyl groups for the site-specific covalent immobilization of protein antibody, anti-troponin I. The hybrid nanocomposite was used as a transducer for biointerfacial impedance sensing for cTnI detection. The results show that the hybrid exhibits a pseudo capacitive behaviour with a maximum phase angle of 49° near 1 Hz, which is due to the inhomogeneous and porous structure of the hybrid composition. The constant phase element of copolymer is 0.61 (n = 0.61), whereas, it is 0.88 (n = 0.88) for the hybrid composites, indicating a comparatively homogeneous microstructure after biomolecular functionalization. The transducer shows a linear change in charge transfer characteristic (R et) on cTnI immunoreaction for spiked human serum in the concentration range of 1.0 pg mL−1–10.0 ng mL−1. The sensitivity of the transducer is 167.8 ± 14.2 Ω cm2 per decade, and it also exhibits high specificity and good reproducibility.

Keywords

Conducting polymer Graphene Carbon nanotube Hybrid Transducer Protein antigen cTnI Electrochemical impedance
Singal, Shobhita, Avanish K. Srivastava, and Rajesh Rajesh. 2016. “Electrochemical Impedance Analysis of Biofunctionalized Conducting Polymer-Modified Graphene-CNTs Nanocomposite for Protein Detection”. Nano-Micro Letters 9 (1):7. https://doi.org/10.1007/s40820-016-0108-2.
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References
  1. J. Gong, X. Miao, T. Zhou, L. Zhang, An enzymeless organophosphate pesticide sensor using Au-nanoparticle decorated graphene hybrid nanosheet as solid-phase extraction. Talanta 85(3), 1344–1349 (2011). doi:10.1016/j.talanta.2011.06.016
  2. J. Lu, I. Do, L.T. Drzal, R.M. Worden, I. Lee, Nanometal-decorated exfoliated graphite nanoplatelet based glucose biosensors with high sensitivity and fast response. ACS Nano 2(9), 1825–1832 (2008). doi:10.1021/nn800244k
  3. X. Sun, L. Qiao, X. Wang, A novel immunosensor based on Au nanoparticles and polyaniline/multiwall carbon nanotubes/chitosan nanocomposite film functionalized interface. Nano-Micro Lett. 5(3), 191–201 (2013). doi:10.1007/BF03353750
  4. D.H. Kim, J.A. Wiler, D.J. Anderson, D.R. Kipke, D.C. Martin, Conducting polymers on hydrogel-coated neural electrode provides sensitive neural recordings in auditory cortex. Acta Biomater. 6(1), 57–62 (2010). doi:10.1016/j.actbio.2009.07.034
  5. S.M. Richardsons, J.L. Hendricks, B. Foster, Polymerization of the conducting polymer poly (3,4-ethylenedioxythiophene) (PEDOT) around living neural cells. Biomaterials 28(8), 1539–1552 (2007). doi:10.1016/j.biomaterials.2006.11.026
  6. A. Ramanavicius, A. Ramanaviciene, A. Malinauskas, Electro-chemical sensors based on conducting polymer-polypyrrole. Electrochim. Acta 51, 6025–6037 (2006). doi:10.1016/j.electacta.2005.11.052
  7. Y.P. Hsiao, Y.W. Su, J.R. Cheng, S.H. Cheng, Electrochemical determination of cysteine based on conducting polymers/gold nanoparticles hybrid nanocomposites. Electrochim. Acta 56(20), 6887–6895 (2011). doi:10.1016/j.electacta.2011.06.031
  8. J.W. Lee, F. Serna, J. Nickels, C.E. Schimdt, Carboxylic acid-functionalized conductive polypyrrole as a bioactive platform for cell adhesion. Biomacromolecules 7(6), 1692–1695 (2006). doi:10.1021/bm060220q
  9. L.F.Q.P. Marchesi, F.R. Simoes, L.A. Pocrifka, E.C. Pereira, Investigation of polypyrrole degradation using electrochemical impedance spectroscopy. J. Phys. Chem. B 115(31), 9570–9575 (2011). doi:10.1021/jp2041263
  10. W. Chen, Z. Lu, C.M. Li, Sensitive human interleukin 5 impedimetricimmunosensor based on polypyrrole-pyrrolepropylic acid-gold nanocomposite. Anal. Chem. 80(22), 8485–8492 (2008). doi:10.1021/ac8012225
  11. D. Wang, W. Hu, Y. Xiong, Y. Xu, C.M. Li, Multifunctionalized reduced graphene oxide-doped polypyrrole/pyrrolepropylic acid nanocomposite impedimetricimmunosensor to ultra-sensitively detect small molecular aflatoxin B1. Biosens. Bioelectron. 63, 185–189 (2015). doi:10.1016/j.bios.2014.06.070
  12. K.S. Novoselov, A.K. Geim, S.V. Morozov, D. Jiang, Y. Zhang, S.V. Dubonos, I.V. Grigorieva, A.A. Firsov, Electric field effect in atomically thin carbon films. Science 306(5696), 666–669 (2004). doi:10.1126/science.1102896
  13. A. Bonnani, A.H. Loo, M. Pumera, Graphene for impedimetric biosensing. Anal. Chem. 37(37), 12–21 (2012). doi:10.1016/j.trac.2012.02.011
  14. Y.M. Lin, C. Dimitrakopoulos, K.A. Jenkins, D.B. Farmer, H.Y. Chiu, A. Grill et al., 100-GHz transistors from wafer-scale epitaxial graphene. Science 327(5966), 662 (2010). doi:10.1126/science.1184289
  15. N. Zhang, M.Q. Yang, S. Liu, Y. Sun, Y.J. Xu, Waltzing with the versatile platform of graphene to synthesize composite photocatalysts. Chem. Rev. 115(18), 10307–10377 (2015). doi:10.1021/acs.chemrev.5b00267
  16. N. Zhang, Y.J. Xu, The endeavour to advance graphene-semiconductor composite-based photocatalysis. Cryst. Eng. Commun. 18(1), 24–37 (2016). doi:10.1039/C5CE01712B
  17. N. Zhang, Y. Zhang, Y.J. Xu, Recent progress on graphene-based photocatalysts: current status and future perspectives. Nanoscale 4(19), 5792–5813 (2012). doi:10.1039/c2nr31480k
  18. S. Paulson, A. Helser, M.B. Nardelli, R.M. Taylor, M. Falvo, R. Superfine, S. Washburn, Tunable resistance of a carbon nanotube-graphite interface. Science 290(5497), 1742–1744 (2000). doi:10.1126/science.290.5497.1742
  19. F.D. Novaes, R. Rurali, P. Ordejon, Electronic transport between graphene layers covalently connected by carbon nanotubes. ACS Nano 4(12), 7596–7602 (2010). doi:10.1021/nn102206n
  20. Y.S. Kim, K. Kumar, F.T. Fisher, E. Yang, Out-of-plane growth of CNTs on graphene for supercapacitor applications. Nanotechnology 23(1), 015301 (2012). doi:10.1088/0957-4484/23/1/015301
  21. K.Y. Hwa, B. Subramani, Synthesis of zinc oxide nanoparticles on graphene-carbon nanotube hybrid for glucose biosensor applications. Biosens. Bioelectron. 62(22), 127–133 (2014). doi:10.1016/j.bios.2014.06.023
  22. B. Kaur, T. Pandiyan, B. Satpati, R. Srivastava, Simultaneous and sensitive determination of ascorbic acid, dopamine, uric acid, and tryptophan with silver nanoparticles-decorated reduced graphene oxide modified electrode. Colloid Surf. B 111(6), 97–106 (2013). doi:10.1016/j.colsurfb.2013.05.023
  23. P. Ammann, M. Pfisterer, T. Fehr, H. Rickli, Raised cardiac troponins; causes extend beyond acute coronary syndromes. Br. Med. J. 328(7447), 1028–1029 (2004). doi:10.1136/bmj.328.7447.1028
  24. S. Ko, B. Kim, S.S. Jo, S.Y. Oh, J.K. Park, Electrochemical detection of cardiac troponin I using a microchip with the surface-functionalized poly (dimethylsiloxane) channel. Biosens. Bioelectron. 23(1), 51–59 (2007). doi:10.1016/j.bios.2007.03.013
  25. A. Qureshi, Y. Gurbuz, J.H. Niazi, Biosensors for cardiac biomarkers detection: a review. Sens. Actuat. B 171–172, 62–76 (2012). doi:10.1016/j.snb.2012.05.077
  26. R.K. Rajesh, A. Paul, Mulchandani, Platinum nanoflowers decorated three-dimensional graphene-carbon nanotubes hybrid with enhanced electrocatalytic activity. J. Power Sources 223(1), 23–29 (2013). doi:10.1016/j.jpowsour.2012.08.088
  27. Y. Li, P. Wang, L. Wang, X. Lin, Over oxidized polypyrrole film directed single-walled carbon nanotubes immobilization on glassy carbon electrode and its sensing applications. Biosens. Bioelectron. 22(12), 3120–3125 (2007). doi:10.1016/j.bios.2007.02.001
  28. B. Rajib, M. Larif, G. Mouhssine, A. Elmidaoui, M.E. Touhami, A. Chaouch, Valorization of polyphenols extracted from olive mill wastewater as ecological corrosion inhibitor on carbon steel in acid medium. Der Pharm. Chem. 8(2), 145–153 (2016)
  29. B. Derkus, M. Ozkan, K.C. Emregul, E. Emregul, Single frequency analysis for clinical immunosensor design. RSC Adv. 6(1), 281–289 (2016). doi:10.1039/C5RA23783A
  30. M. Bart, E.C.A. Stigter, H.R. Stapert, G.J. Jong, W.P. Bennekom, On the response of a labl-free interferon –γ immunosensor utilizing electrochemical impedance spectroscopy. Biosens. Bioelectron. 21(1), 49–59 (2005). doi:10.1016/j.bios.2004.10.009
  31. A. Periyakaruppan, R.P. Gandhiraman, M. Meyyappan, J.E. Koehne, Label-free detection of cardiac troponin-I using carbon nanofiber based nanoelectrode array. Anal. Chem. 85(8), 3858–3863 (2013). doi:10.1021/ac302801z
  32. Z.R. Guo, C.R. Gu, X. Fan, Z.P. Bian, H.F. Wu, D. Yang, N. Gu, J.N. Zhang, Fabrication of anti-human cardiac troponin I immunogold nanorods for sensing acute myocardial damage. Nanoscale Res. Lett. 4(12), 1428–1433 (2009). doi:10.1007/s11671-009-9415-6
  33. A.J.S. Ahammad, Y.H. Choi, K. Koh, J.H. Kim, J.J. Lee, M. Lee, Electrochemical detection of cardiac biomarker troponin I at gold nanoparticle-modified ITO electrode by using open circuit potential. Int. J. Electrochim. Sci. 6(6), 1906–1916 (2011)
  34. A.A. Shumkov, E.V. Suprun, S.Z. Shatinina, A.V. Lisitsa, V.V. Shumyantseva, A.I. Archakov, Gold and silver nanoparticles for electrochemical detection of cardiac troponin I based on stripping voltammetry. J. Bionanosci. 3(2), 216–222 (2013). doi:10.1007/s12668-013-0090-9
  35. W.Y. Wu, Z.P. Bian, W. Wang, W. Wang, J.J. Zhu, PDMS gold nanoparticle composite film-based silver enhanced colorimetric detection of cardiac troponin I. Sens. Actuat. B 147(1), 298–303 (2010). doi:10.1016/j.snb.2010.03.027
  36. Y. Ryu, Z. Jin, M.S. Kang, H.S. Kim, Increase in the detection sensitivity of a lateral flow assay for a cardiac marker by oriented immobilization of antibody. Biochip J. 5, 193–198 (2011). doi:10.1007/s13206-011-5301-2
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