Label-free colorimetric estimation of proteins using nanoparticles of silver

Siddhartha Shrivastava; Debabrata Dash

doi:10.1007/BF03353636

Issue

Vol. 2 No. 3 (2010)

Issue Published : Jul 1, 2010

Label-free colorimetric estimation of proteins using nanoparticles of silver

https://doi.org/10.1007/BF03353636

Siddhartha Shrivastava

Department of Biochemistry, Institute of Medical Sciences, Banaras Hindu University, Varanasi-221005 (U.P.) India

Debabrata Dash

Department of Biochemistry, Institute of Medical Sciences, Banaras Hindu University, Varanasi-221005 (U.P.) India

Corresponding Author: Debabrata Dash

ddass@satyam.net.in

Nano-Micro Letters, Vol. 2 No. 3 (2010), Article Number: 164-168
Article Published : Sep 16, 2010

Abstract

Metallic nanoparticles have received considerable attention in bioassays and diagnostics due to their unique surface plasmon resonance (SPR) properties. Gold nanoparticles have been employed for the development of SPR-based colorimetric bioassays. In the present report we have described a sensitive colorimetric approach for estimation of proteins, within a detection limit of 10∼80 μg/mL, using unmodified silver nanoparticles. Besides the common advantages of colorimetric assay such as simplicity, high sensitivity, and low cost, our method has a label-free design and provides an important and attractive alternative to classical sensing probes and systems. The present work will contribute to the development of nanotechnology-based diagnostic tools.

Keywords

Silver nanoparticles Agglutination Surface plasmon resonance Colorimetric estimation Bovine serum albumin Immunoglobulin

Shrivastava, Siddhartha, and Debabrata Dash. 2010. “Label-Free Colorimetric Estimation of Proteins Using Nanoparticles of Silver”. Nano-Micro Letters 2 (3):164-68. https://doi.org/10.1007/BF03353636.

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References

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Y. Lu and J. Liu, Acc. Chem. Res. 40, 315 (2007). doi:10.1021/ar600053g
S. K. Singh, S. Shrivastava, M. K. Nayak, A. S. K. Sinha, M. V. Jagannadham and D. Dash, J. Bionanosci. 3, 88 (2009).
S. Shrivastava, T. Bera, A. Roy, G. Singh, P. Ramachandrarao and D. Dash, Nanotechnology 18, 225103 (2007). doi:10.1088/0957-4484/18/22/225103
S. Shrivastava, T. Bera, S. K. Singh, G. Singh, P. Ramachandrarao and D. Dash, ACS Nano 3, 1357 (2009). doi:10.1021/nn900277t
Y. Chen, J. Aveyard and R. Wilson, Chem. Commun. 24, 2804 (2004). doi:10.1039/b411181h
S. Liu, Z. Zhang and M. Han, Anal. Chem. 77, 2595 (2005). doi:10.1021/ac0482864
K. Yoosaf, B. I. Ipe, C. H. Suresh and K. G. Thomas, J. Phys. Chem. C 111, 12839 (2007). doi:10.1021/jp073923q
C. L. Schofield, A. H. Haines, R. A. Field and D. A. Russell, Langmuir 22, 6707 (2006). doi:10.1021/la060288r
P. Pandey, S. P. Singh, S. K. Arya, V. Gupta, M. Datta, S. Singh and B. D. Malhotra, Langmuir 23, 3333 (2007). doi:10.1021/la062901c
D. C. Carter and J. X. Ho, Adv. Protein Chem. 45, 153 (1994). doi:10.1016/S0065-3233(08)60640-3
S. Stamler, D. J. Singel and J. Loscalzo, Science 258, 1898 (1992). doi:10.1126/science.1281928
J. Wang, L. Wang, X. Liu, Z. Liang, S. Song, W. Li, G. Li and C. Fan, Adv. Mater. 19, 3943 (2007). doi:10.1002/adma.200602256

References

R. Elghanian, J. J. Storhoff, R. C. Mucic, R. L. Letsinger and C. A. Mirkin, Science 277, 1078 (1997). doi:10.1126/science.277.5329.1078

H. Li and L. Rothberg, Proc. Natl. Acad. Sci. USA 101, 14036 (2004). doi:10.1073/pnas.0406115101

H. Li and L. Rothberg, J. Am. Chem. Soc. 126, 10958 (2004). doi:10.1021/ja048749n

M. Mascini, G. G. Guilbaut and S. J. Lebrun, Anal. Lett. 40, 1386 (2007). doi:10.1080/00032710701327062

C. C. Huang, Y. F. Huang, Z. Cao, W. Tan and H. T. Chang, Anal. Chem. 77, 5735 (2005). doi:10.1021/ac050957q

J. S. Lee, P. A. Ulmann, M. S. Han and C. A. Mirkin, Nano Lett. 8, 529 (2008). doi:10.1021/nl0727563

Z. Wang, R. Lévy, D. G. Fernig and M. Brust, J. Am. Chem. Soc. 128, 2214 (2006). doi:10.1021/ja058135y

H. Wei, C. Chen, B. Han and Wang, Anal. Chem. 80, 7051 (2008). doi:10.1021/ac801144t

Liu and Y. Lu, J. Am. Chem. Soc. 125, 6642 (2003). doi:10.1021/ja034775u

Y. Lu and J. Liu, Acc. Chem. Res. 40, 315 (2007). doi:10.1021/ar600053g

S. K. Singh, S. Shrivastava, M. K. Nayak, A. S. K. Sinha, M. V. Jagannadham and D. Dash, J. Bionanosci. 3, 88 (2009).

S. Shrivastava, T. Bera, A. Roy, G. Singh, P. Ramachandrarao and D. Dash, Nanotechnology 18, 225103 (2007). doi:10.1088/0957-4484/18/22/225103

S. Shrivastava, T. Bera, S. K. Singh, G. Singh, P. Ramachandrarao and D. Dash, ACS Nano 3, 1357 (2009). doi:10.1021/nn900277t

Y. Chen, J. Aveyard and R. Wilson, Chem. Commun. 24, 2804 (2004). doi:10.1039/b411181h

S. Liu, Z. Zhang and M. Han, Anal. Chem. 77, 2595 (2005). doi:10.1021/ac0482864

K. Yoosaf, B. I. Ipe, C. H. Suresh and K. G. Thomas, J. Phys. Chem. C 111, 12839 (2007). doi:10.1021/jp073923q

C. L. Schofield, A. H. Haines, R. A. Field and D. A. Russell, Langmuir 22, 6707 (2006). doi:10.1021/la060288r

P. Pandey, S. P. Singh, S. K. Arya, V. Gupta, M. Datta, S. Singh and B. D. Malhotra, Langmuir 23, 3333 (2007). doi:10.1021/la062901c

D. C. Carter and J. X. Ho, Adv. Protein Chem. 45, 153 (1994). doi:10.1016/S0065-3233(08)60640-3

S. Stamler, D. J. Singel and J. Loscalzo, Science 258, 1898 (1992). doi:10.1126/science.1281928

J. Wang, L. Wang, X. Liu, Z. Liang, S. Song, W. Li, G. Li and C. Fan, Adv. Mater. 19, 3943 (2007). doi:10.1002/adma.200602256

Issue

Vol. 2 No. 3 (2010)

Label-free colorimetric estimation of proteins using nanoparticles of silver

Corresponding Author: Debabrata Dash

Abstract

Keywords

Download Citation

References

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