Issue

Vol. 4 No. 4 (2012)

Nanobiocomposite Electrochemical Biosensor Utilizing Synergic Action of Neutral Red Functionalized Carbon Nanotubes

https://doi.org/10.1007/BF03353718
D. R. Shobha Jeykumari
Department of Chemistry, Women’s Christian college, Nungambakkam, Chennai, 600 006, TN, India
R. Kalaivani
Department of Chemistry, School of Basic Sciences ,VELS University, Pallavaram, Chennai, 600 117, TN, India
S. Sriman Narayanan
Department of Analytical Chemistry, School of Chemical Sciences, University of Madras, Guindy Campus, Chennai, 600 025, TN, India

Corresponding Author: S. Sriman Narayanan

sriman55@gmail.com

Nano-Micro Letters, Vol. 4 No. 4 (2012), Article Number: 220-227

Abstract

An amperometric hydrogen peroxide biosensor using a nanobiocomposite based on neutral red modified carbon nanotubes and co-immobilized glucose oxidase and horseradish peroxidase is reported. Modification of the nanobiocomposite electrode with neutral red resulted in a sensitive, low-cost and reliable H2O2 sensor. The use of carbon nanotubes, as the conductive part of the composite, facilitated fast electron transfer rates. The biosensor was characterized for the influence of pH, potential and temperature. A remarkable feature of the biosensor is the detection of H2O2 at low applied potentials where the noise level and interferences are minimal. The sensor has a fast steady-state measuring time of 10 s with a quick response (2 s). The biosensor showed a linear range from 15 nM to 45 mM of H2O2 and a detection limit of 5 nM. Nafion, which is used as a binder, makes the determination free from other electroactive substances. The repeatability, reproducibility, stability and analytical performance of the sensor are very good.

Keywords

Multiwalled carbon nanotubes Neutral red Glucose oxidase Horseradish peroxidase Nafion Nanobiocomposite biosensor
Jeykumari, D. R. Shobha, R. Kalaivani, and S. Sriman Narayanan. 2012. “Nanobiocomposite Electrochemical Biosensor Utilizing Synergic Action of Neutral Red Functionalized Carbon Nanotubes”. Nano-Micro Letters 4 (4):220-27. https://doi.org/10.1007/BF03353718.
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