Issue

Vol. 7 No. 1 (2015)

High-Throughput Screening of Nanoparticle-Stabilizing Ligands: Application to Preparing Antimicrobial Curcumin Nanoparticles by Antisolvent Precipitation

https://doi.org/10.1007/s40820-014-0020-6
Ilya Shlar
Institute of Postharvest and Food Sciences, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Elena Poverenov
Institute of Postharvest and Food Sciences, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Yakov Vinokur
Institute of Postharvest and Food Sciences, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Batia Horev
Institute of Postharvest and Food Sciences, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Samir Droby
Institute of Postharvest and Food Sciences, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Victor Rodov
Institute of Postharvest and Food Sciences, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel

Corresponding Author: Victor Rodov

vrodov@agri.gov.il

Nano-Micro Letters, Vol. 7 No. 1 (2015), Article Number: 68-79

Abstract

Water-dispersible curcumin nanoparticles were prepared by bottom-up antisolvent precipitation approach. A new high-throughput screening technique was developed for selecting appropriate ligands stabilizing the nanoparticles in aqueous medium and improving their performance. The initial set of twenty-eight potential stabilizing ligands was evaluated based on their capacity to improve curcumin dispersibility in aqueous medium. The performance of four promising ligands (amino acid proline, polyphenol tannic acid, polycation Polyquaternium 10, and neutral polymer polyvinylpyrrolidone) was tested in ultrasound-aided antisolvent precipitation trials. Using the selected stabilizing ligands diminished the average particle size from ca. 1,200 to 170–230 nm, reduced their dispersity, improved stability, and allowed reaching curcumin concentration of up to 1.4 mM in aqueous medium. Storage stability of the aqueous nanodispersions varied from 2 days to 2 weeks, depending on stabilizing ligand. Studying the effects of ionic strength and pH on size and ζ-potential of the particles suggested that electrostatic forces and hydrophobic interactions could be the major factors affecting their stability. The ligand-protected nanoparticles showed minimal inhibitory concentration of 400 or 500 µM toward Escherichia coli. We suggest that the presented screening approach may be useful for preparing nanoparticles of various poorly water-soluble bioactive materials.

Keywords

Nanoparticles Curcumin Antisolvent precipitation Stabilizing ligands High-throughput screening Antimicrobial E. coli
Shlar, Ilya, Elena Poverenov, Yakov Vinokur, Batia Horev, Samir Droby, and Victor Rodov. 2014. “High-Throughput Screening of Nanoparticle-Stabilizing Ligands: Application to Preparing Antimicrobial Curcumin Nanoparticles by Antisolvent Precipitation”. Nano-Micro Letters 7 (1):68-79. https://doi.org/10.1007/s40820-014-0020-6.
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