Synthesis of silver nanoparticles by Lactobaciluus acidophilus 01 strain and evaluation of its in vitro genomic DNA toxicity

S. Karthick Raja Namasivayam; E. Kumar Gnanendra; R. Reepika

doi:10.1007/BF03353635

Issue

Vol. 2 No. 3 (2010)

Issue Published : Jul 1, 2010

Synthesis of silver nanoparticles by Lactobaciluus acidophilus 01 strain and evaluation of its in vitro genomic DNA toxicity

https://doi.org/10.1007/BF03353635

S. Karthick Raja Namasivayam

Department of Biotechnology, Sathyabama University, Chennai 600119, Tamil Nadu, India

E. Kumar Gnanendra

Department of Biotechnology, Sathyabama University, Chennai 600119, Tamil Nadu, India

R. Reepika

Department of Biotechnology, Sathyabama University, Chennai 600119, Tamil Nadu, India

Corresponding Author: S. Karthick Raja Namasivayam

biologiask@gmail.com

Nano-Micro Letters, Vol. 2 No. 3 (2010), Article Number: 160-163
Article Published : Aug 24, 2010

Abstract

Silver nanoparticles synthesized by dried biomass of Lactobaciluus acidophilus 01 strain was evaluated against toxicity of genomic DNA isolated from bacteria (E. coli) fungi (Beauveria bassiana) algae (Seenedesmus acutus) and human blood adopting standard condition was discussed in the present study. Synthesized silver nanoparticles were characterized by UV-Vis spectroscopy and SEM. The UV-Vis spectroscopy revealed the formation of silver nanoparticles by yielding the typical silver plasmon absorption maxima at 430 nm and SEM micrograph indicates the uniform spherical particles within the size range of 45∼60 nm. The energy dispersive X-ray spectroscopy (EDX) of the nanoparticle confirmed the presence of elemental silver signal as strong peak. The above synthesized silver nanoparticles didn’t cause any toxic effect on all the tested genomic DNA at all tested concentrations which reveals nil genomic nanoparticles induced toxicity.

Keywords

Lactobacillus acidophilus Silver nanoparticles Antibacterial activity Biosynthesis

Namasivayam, S. Karthick Raja, E. Kumar Gnanendra, and R. Reepika. 2010. “Synthesis of Silver Nanoparticles by Lactobaciluus Acidophilus 01 Strain and Evaluation of Its in Vitro Genomic DNA Toxicity”. Nano-Micro Letters 2 (3):160-63. https://doi.org/10.1007/BF03353635.

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References

Ahmad, P. Mukharjee, S. Senapati, D Mandal and M. Sastry, Colloids Surf. B 28, 313 (2008). doi:10.1016/S0927-7765(02)00174-1.
D. Cui and H. Gao, Biotechnol. Prog. 19, 683 (2003). doi:10.1021/bp025791i.
L. Dubas, T. Kulsiriwiwat and K. Samningjam. Acta. Metallurgica Slovaca 13, 1559 (2007).
OC. Farokhzad, J. Cheng, B. A. Teply, Sherifi, S. Jon, P. W. Kantoff, JP Richie and R. Langer, Proc. Natl. Acad. Sci. USA. 103, 6315 (2008). doi:10.1073/pnas.0601755103.
R. Feynman, Science 254, 1300 (1991). doi:10.1126/science.254.5036.1300.
C, Greulich, Kittler M. Epple, G. Muhr and Köller, Arch. Surg. 394, 49 (2001).
M. James, NNIN REU Research Accomplishments 146, 147 (2004).
T. Klaus, R. Joerger, E. Olsson and C. G. Granqvist, Trends Biotechnol. 19, 15 (2001). doi:10.1016/S0167-7799(00)01514-6.
M. Kumar and R. M. Sastry, Nano Lett. 1, 151 (2001).
M. Kowshik, A. Ahmad, D. Mandal and S. Senapati, Nanotechnology 14, 95 (2003). doi:10.1088/0957-4484/14/ 1/321.
P. D. Marcato, G. I. H. DeSiyza, O. L. Alves, E. Esposito and N. Duran, 4th Mercosur Congress on Process Systems Engineering (2008).
G. Maribel Guzman, Jean Dille and Stephan Gode, World Academy of Science, Eng. Technol. 43, 17 (2008).
P. Mehrbod, N. Motamed, M. Tabatabaian, R. Soleimani Estyar and M. T. Kheri, DARU. 17,121 (2009).
S. Minaeian, A. R. Shahverdi, A. S. Nohi and H. R. Shahverdi, J. Sci. I. A. U. 17, 31 (2009).
K. Roy, H. Q. Mao, S. K. Huang and K. W. Leong, Nat. Med. 5, 387 (1999). doi:10.1038/7385.
M. V. Rutberg, V. A. Dubina, E. V. Kolikov, E. V. Moiseenko, N. M. Ignateva, Volkov, V. N. Snetov and A. Yu. Stogov, Biochem. Biophys. 421, 193 (2008).
E. Sachlos, D. Gotora and J. T. Czernuszka, Tissue Eng. 12, 2479 (2006). doi:10.1089/ten.2006.12.2479.
I. H. Sadowski Maliszewska, B. Grochowalska, J. Polowczyk and T. Kozlecki, Mater. Science-Poland 26, 213 (2008).

References

Ahmad, P. Mukharjee, S. Senapati, D Mandal and M. Sastry, Colloids Surf. B 28, 313 (2008). doi:10.1016/S0927-7765(02)00174-1.

D. Cui and H. Gao, Biotechnol. Prog. 19, 683 (2003). doi:10.1021/bp025791i.

L. Dubas, T. Kulsiriwiwat and K. Samningjam. Acta. Metallurgica Slovaca 13, 1559 (2007).

OC. Farokhzad, J. Cheng, B. A. Teply, Sherifi, S. Jon, P. W. Kantoff, JP Richie and R. Langer, Proc. Natl. Acad. Sci. USA. 103, 6315 (2008). doi:10.1073/pnas.0601755103.

R. Feynman, Science 254, 1300 (1991). doi:10.1126/science.254.5036.1300.

C, Greulich, Kittler M. Epple, G. Muhr and Köller, Arch. Surg. 394, 49 (2001).

M. James, NNIN REU Research Accomplishments 146, 147 (2004).

T. Klaus, R. Joerger, E. Olsson and C. G. Granqvist, Trends Biotechnol. 19, 15 (2001). doi:10.1016/S0167-7799(00)01514-6.

M. Kumar and R. M. Sastry, Nano Lett. 1, 151 (2001).

M. Kowshik, A. Ahmad, D. Mandal and S. Senapati, Nanotechnology 14, 95 (2003). doi:10.1088/0957-4484/14/ 1/321.

P. D. Marcato, G. I. H. DeSiyza, O. L. Alves, E. Esposito and N. Duran, 4th Mercosur Congress on Process Systems Engineering (2008).

G. Maribel Guzman, Jean Dille and Stephan Gode, World Academy of Science, Eng. Technol. 43, 17 (2008).

P. Mehrbod, N. Motamed, M. Tabatabaian, R. Soleimani Estyar and M. T. Kheri, DARU. 17,121 (2009).

S. Minaeian, A. R. Shahverdi, A. S. Nohi and H. R. Shahverdi, J. Sci. I. A. U. 17, 31 (2009).

K. Roy, H. Q. Mao, S. K. Huang and K. W. Leong, Nat. Med. 5, 387 (1999). doi:10.1038/7385.

M. V. Rutberg, V. A. Dubina, E. V. Kolikov, E. V. Moiseenko, N. M. Ignateva, Volkov, V. N. Snetov and A. Yu. Stogov, Biochem. Biophys. 421, 193 (2008).

E. Sachlos, D. Gotora and J. T. Czernuszka, Tissue Eng. 12, 2479 (2006). doi:10.1089/ten.2006.12.2479.

I. H. Sadowski Maliszewska, B. Grochowalska, J. Polowczyk and T. Kozlecki, Mater. Science-Poland 26, 213 (2008).